PPB021
SSPE Buffer
pH 7.4, pHast Pack™, powder
Synonym(s):
1X SSPE Hybridization buffer, 1X SSPE buffer, SSPE Hybridization buffer, Saline-Sodium Phosphate-EDTA Buffer
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About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
Related Categories
Application
Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH2PO4, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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