C1144

Chlorazol Black

• Synonym(s): 4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38
• Empirical Formula (Hill Notation): C₃₄H₂₅N₉Na₂O₇S₂
• CAS Number: 1937-37-7
• Molecular Weight: 781.73
• Colour Index Number: 30235
• EC Number: 217-710-3
• MDL number: MFCD00066363
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892361
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 200
• composition: Dye content, ≥45% (based on Nitrogen)
• color: dark brown to black
• solubility: H₂O: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1
• InChI: 1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;
• InChI key: XRPLBRIHZGVJIC-UVHCWRHYSA-L

Description

General description

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin. It also helps in distinguishing fungal bodies from artefacts due to chitin staining. It is also used as an auxiliary stain for chromosomes.

Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H319 - H350 - H361d
• Precautionary Statements: P202 - P264 - P280 - P305 + P351 + P338 - P308 + P313 - P337 + P313
• Hazard Classifications: Carc. 1B - Eye Irrit. 2 - Repr. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves