A8654

Bovine Serum Albumin

dimer mol wt ~132 kDa

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC Code: 12352202
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 200
• Assay: 0.9-1.4 mg per vial protein basis (biuret)
• form: lyophilized powder
• mol wt: dimer ~132 kDa; monomer ~66 kDa; ~66 kDa
• purified by: cold ethanol fractionation
• origin: USA origin
• pH: 5.2
• storage temp.: −20°C

Description

Application

Marker for non-denaturing PAGE

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves