93313

Trizma^® hydrochloride buffer solution

BioUltra, for molecular biology, pH 7.4

• Synonym(s): Tris(hydroxymethyl)aminomethane hydrochloride buffer solution
• Beilstein: 3675235
• MDL number: MFCD00012590
• UNSPSC Code: 12000000
• eCl@ss: 32129211
• PubChem Substance ID: 329770223
• NACRES: ND.02

Properties

• grade: for molecular biology
• Quality Level: 100
• product line: BioUltra
• form: liquid
• concentration: 1.0 M tris-HCl
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 7.4±0.1 (25 °C)
• density: 1.05 g/mL at 20 °C
• cation traces: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.04
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

• Reduced glutathione inhibits rabbit and rat skeletal muscle lactate dehydrogenase and prevents dinitrophenol induced extracellular acidification by an epithelial cell line.: This study investigates how Trizma^® hydrochloride buffer solution maintains pH stability, critical for accurately assessing the biochemical interactions in muscle and epithelial cells (Wimberger et al., 1997).

Other Notes

Buffer component (SET buffer) in the preparation of RNA

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves