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carrier
PAA carrier
label
FITC labeled
product line
FP-Series
Assay
95% (H-NMR)
form
lyophilized powder
mol wt
~20,000 Da
manufacturer/tradename
GlycoNZ
extent of labeling
1% Biotin per 20 mole % Poly[N-(2-hydroxyethyl)acrylamide-co-N-acrylamide] ligand density
technique(s)
flow cytometry: suitable
probe type
trisaccharide
probe presentation
polymeric
storage temp.
−20°C
General description
FP-Series: Fluorescein-Labeled Polymeric Probes
These glycoconjugate probes are designed to reveal lectins, including selectins, directly on cell membranes, organelles, and in cytoplasm. The low fluorescein content ensures that probe solubility in water is not affected, but still allows reliable probe detection. The affinity of the probes is 102 to 105 times higher than that of corresponding free sugars.
PAA is poly[N-(2-hydroxyethyl)acrylamide], a flexible polymer chain which serves as an additional spacer. Used as a molecular weight carrier, PAA demonstrates an absence of non-specific interaction with cells, and is stable to chemical and proteolytic action.
These glycoconjugate probes are designed to reveal lectins, including selectins, directly on cell membranes, organelles, and in cytoplasm. The low fluorescein content ensures that probe solubility in water is not affected, but still allows reliable probe detection. The affinity of the probes is 102 to 105 times higher than that of corresponding free sugars.
PAA is poly[N-(2-hydroxyethyl)acrylamide], a flexible polymer chain which serves as an additional spacer. Used as a molecular weight carrier, PAA demonstrates an absence of non-specific interaction with cells, and is stable to chemical and proteolytic action.
Application
Glycoconjugates are used to study the functions of carbohydrates, carbohydrate-binding proteins in biological processes, and on the discovery of new carbohydrate-mediated interactions.
Preparation Note
Purity: synthetic ω-aminoalkyl glycosides used for coupling with the polymer have a purity of >95% (HPLC and 1H-NMR)
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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