AGRLE-RO
Roche
Agarose LE
low electroendosmosis
Synonym(s):
agarose
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About This Item
UNSPSC Code:
12352200
biological source
algae (seaweed)
form
powder
packaging
pkg of 100 g (11685660001)
pkg of 500 g (11685678001)
manufacturer/tradename
Roche
EEO
0.05 -0.13
transition temp
gel point 36 °C ±1.5
gel strength
>=2,500 g/cm2
foreign activity
Dnase, none detected
Rnase, none detected
shipped in
ambient
storage temp.
20-25°C
General description
Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.
Application
Agarose LE has been used for:
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
- analyzing fragments between 0.2 and 15 kb
- analysis of PCR products
- examination of restriction endonucleases
- digests of plasmid, cosmid, and λ phage DNA
- electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
- in combination with ethidium bromide in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.
Quality
Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)
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