10165948001
Roche
RNA, MS2
from bacteriophage MS2
Synonym(s):
RNA, ribonucleic acid
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106311
Recommended Products
biological source
bacteriophage MS2
Quality Level
form
solution
mol wt
~1200 kDa
packaging
pkg of 500 μL (10 A260 units)
manufacturer/tradename
Roche
technique(s)
RNA extraction: suitable
color
colorless
solubility
water: miscible
storage temp.
−20°C (−15°C to −25°C)
Related Categories
General description
RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.
Application
RNA, MS2 has been used:
- as a standard to assess RNA quantification methods
- in RNA extraction
- in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
- for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
- for structural and functional studies.
- to stabilize RNA during cDNA synthesis
- to improve RNA yield during RNA extraction or isolation
Quality
Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.
Sequence
Chain Length 3,569 nucleotides
Preparation Note
Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Rom S Leidner et al.
PloS one, 8(3), e57841-e57841 (2013-03-09)
Genome-wide platforms for high-throughput profiling of circulating miRNA (oligoarray or miR-Seq) offer enormous promise for agnostic discovery of circulating miRNA biomarkers as a pathway for development in breast cancer detection. By harmonizing data from 15 previous reports, we found widespread
Thomas C Roberts et al.
Biological procedures online, 16(1), 5-5 (2014-03-19)
MicroRNAs (miRNAs) are short RNA molecules which regulate gene expression in eukaryotic cells, and are abundant and stable in biofluids such as blood serum and plasma. As such, there has been heightened interest in the utility of extracellular miRNAs as
Roman Aranda et al.
Analytical biochemistry, 387(1), 122-127 (2009-05-21)
Quantification of RNA is essential for various molecular biology studies. In this work, three quantification methods were evaluated: ultraviolet (UV) absorbance, microcapillary electrophoresis (MCE), and fluorescence-based quantification. Viral, bacterial, and eukaryotic RNA were measured in the 500 to 0.05-ng microl(-1)
Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative
Shalini Das Gupta et al.
Scientific reports, 10(1), 9012-9012 (2020-06-04)
Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service