MABT1444

Anti-Tetranectin/CLEC3B Antibody, clone mAb10

• Synonym(s): Anti-CLEC3B Antibody, CLEC3B Detection Antibody, Clone mAb10 Anti-Tetranectin
• UNSPSC Code: 12352203
• NACRES: NA.74

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: mAb10, monoclonal
• mol wt: calculated mol wt 22.54 kDa; observed mol wt ~25 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; dot blot: suitable; western blot: suitable
• isotype: IgG2bκ
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_003269
• UniProt accession no.: P05452
• shipped in: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CLEC3B(7123)

Description

General description

Tetranectin (UniProt: P05452; also known as TN, C-type lectin domain family 3 member B, Plasminogen kringle 4-binding protein) is encoded by the CLEC3B (also known as TNA) gene (Gene ID: 7123) in human. Tetranectin is a tetrameric plasminogen-binding protein that is synthesized with a signal peptide (aa 1-21), which is subsequently removed in the mature form that is composed of three identical, noncovalently linked subunits each of 181 amino acids. The polypeptide consists of a short a-helical segment (aa 47-72) at the amino terminus that is involved in trimerization, followed by a C-type lectin domain (aa 77-198). It is present in both serum and the extracellular matrix and is expressed most abundantly in the lung. The blood concentrations of tetranectin in healthy adults are about 10-12 mg/mL. However, in septic subjects its levels are significantly reduced and supplementation with exogenous tetranectin confers dose-dependent protection against lethal endotoxemia and sepsis. Its levels are shown to be significantly reduced in patients with cardiac failure. Hence, it is also used as a biomarker for risk of heart failure. (Ref.: Chen, W., et al. (2020). Sci. Transl. Med. 12(539); eaaz3833; McDonald, K., et al. (2020). Sci. Rep. 10; 7507).

Specificity

Clone mAb10 is a mouse monoclonal antibody that detects Tetranectin. It targets an epitope within 24 amino acids from the C-terminal half.

Immunogen

Purified human Tetranectin.

Application

Quality Conrol Testing

Evaluated by Western Blotting in Human lung tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tetranectin/CLEC3B in Human lung tissue lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Tetranectin/CLEC3B in Western Blotting applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).

Dot Blot: A representative lot detected Tetranectin/CLEC3B in Dot Blot applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).

ELISA Analysis: A representative lot detected Tetranectin/CLEC3B in ELISA applications (Chen, W., et al. (2020). Sci Transl Med. 12(539):eaaz3833).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Tetranectin/CLEC3B, clone mAb10, Cat. No. MABT1444, is a mouse monoclonal antibody that detects Tetranectin and is tested for use in Dot Blot, ELISA, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable