MABN717

Anti-WNT10B Antibody, clone 5A7

ascites fluid, clone 5A7, from mouse

• Synonym(s): Protein Wnt-10b, Protein Wnt-12
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 5A7, monoclonal
• species reactivity: human
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_003385
• UniProt accession no.: O00744
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... WNT10B(7480)

Description

General description

Wingless-type MMTV integration site family member 10B (WNT10B), also known as Protein Wnt-12 (WNT12), consists of structurally related secreted signaling proteins implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is detected in most adult tissues, with the highest levels found in heart and skeletal muscle, and lower levels found in brain. WNT10B may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. Defects in WNT10B are linked to split-hand/foot malformation type 6 (SHFM6).

Immunogen

Purified recombinant fragment of human WNT10B expressed in E. coli.

Application

Anti-WNT10B Antibody, clone 5A7 is a highly specific mouse monoclonal antibody, that targets Wnt & has been tested in IHC, western blotting & Immunofluorescence.

Immunohistochemistry Analysis: A 1:500-1,000 dilution from a representative lot detected WNT10B in human kidney and liver tissues.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT10B in PANC-1 cells.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WNT10B in 10 µg of HeLa cell lysate.

Target description

~47 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable