MABE967

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1

clone 11E10.1, from mouse

• Synonym(s): DNA polymerase delta catalytic subunit, DNA polymerase delta p125, DNA polymerase subunit delta p125
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 11E10.1, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001243778
• UniProt accession no.: P28340
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... POLD1(5424)

Description

General description

DNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human. DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ɛ. While Pol α initiates DNA synthesis, Pol δ and Pol ɛ replace Pol α after primer extension and perform the bulk of DNA replication. Pol δ and Pol ɛ are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA). Both POLD1 and POLE contain an exonuclease domain (a.a. 268–471 of POLE and 304–517 of POLD1) that proof-reading capability during DNA replication. In addition to DNA synthesis, Pol ɛ and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR). Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP). Tumors with EDMs exhibits dramatically increased base substitutions.

Immunogen

GST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.

Application

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DNA polymerase delta p125 in 10 µg of Ramos cell lysate.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA polymerase delta p125 in 10 µg of Raji cell lysate.

Target description

~125 kDa observed.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable