MAB6021
Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5
culture supernatant, clone 1G5, Chemicon®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
1G5, monoclonal
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Cyp11B2(13072)
rat ... Cyp11B2(24294)
General description
Aldosterone synthase (or 18-hydroxylase) is a steroid hydroxylase cytochrome P450 oxidase enzyme involved in the generation of aldosterone. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. (Pascoe, L, et al. (1992) "Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency." PNAS, USA. 89:496-5000).
Specificity
Rat cytochrome p450 aldosterone synthase
Immunogen
Aldo-1-MAP (aa 175-192) (KVRQNARGSLTMDVQQSL-MAP)
Application
This Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5 is validated for use in ELISA, IC, IH(P), WB for the detection of Cytochrome P450 Aldosterone Synthase.
Western blot: recognizes ~48kDa band in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable.
Immunohistochemistry: 1:50-1:200 , ABC, enzymatic detection; clone works best with STF fixed tissues that are paraffin embedded. STF is available from Streck Laboratories, www.streck.com. Formalin fixation has been successfully reported but working dilutions are much lower (see Fujisawa, et al). Recommended counter stain is Gill′s Haematoxylin.
Immunocytochemistry: acetone fixed cells, 1:20-1:200.
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:50-1:200 , ABC, enzymatic detection; clone works best with STF fixed tissues that are paraffin embedded. STF is available from Streck Laboratories, www.streck.com. Formalin fixation has been successfully reported but working dilutions are much lower (see Fujisawa, et al). Recommended counter stain is Gill′s Haematoxylin.
Immunocytochemistry: acetone fixed cells, 1:20-1:200.
ELISA
Optimal working dilutions must be determined by end user.
Physical form
Unpurified tissue culture supernatant containing 50% glycerol and 0.1% sodium azide as a preservative.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Role of the renin-angiotensin-aldosterone system in the enhancement of salt sensitivity caused by prenatal protein restriction in stroke-prone spontaneously hypertensive rats.
Otani, L; Sugimoto, N; Kaji, M; Murai, M; Chang, SJ; Kato, H; Murakami, T
The Journal of Nutritional Biochemistry null
Local renal aldosterone system and its regulation by salt, diabetes, and angiotensin II type 1 receptor.
Xue, C; Siragy, HM
Hypertension null
Mineralocorticoid receptor antagonist spironolactone prevents pig serum-induced hepatic fibrosis in rats.
Fujisawa, G; Muto, S; Okada, K; Kusano, E; Ishibashi, S
Translational Research null
Ana M Briones et al.
Hypertension (Dallas, Tex. : 1979), 59(5), 1069-1078 (2012-04-12)
We reported aldosterone as a novel adipocyte-derived factor that regulates vascular function. We aimed to investigate molecular mechanisms, signaling pathways, and functional significance of adipocyte-derived aldosterone and to test whether adipocyte-derived aldosterone is increased in diabetes mellitus-associated obesity, which contributes
Xiaoli Zhou et al.
Journal of the American Society of Nephrology : JASN, 27(6), 1765-1776 (2015-10-17)
Deficiency of the antiaging gene Klotho (KL) induces renal damage and hypertension through unknown mechanisms. In this study, we assessed whether KL regulates expression of CYP11B2, a key rate-limiting enzyme in aldosterone synthesis, in adrenal glands. We found that haplodeficiency
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