ESG1106
ESGRO® Recombinant Mouse LIF Protein
ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml
Synonym(s):
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF
About This Item
Recommended Products
Quality Level
Assay
>95% (active component mLIF, SDS-PAGE)
form
liquid
manufacturer/tradename
Chemicon®
concentration
1000000 units/mL
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
mouse ... LIF(16878)
General description
Application
SUGGESTED PROTOCOLS:
ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3).
At the recommended concentration 107 units of ESGRO Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO Supplement is sufficient for 1.0 L of tissue culture media.
Physical form
Preparation Note
Storage and Stability
Analysis Note
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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