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ABS2130M

Sigma-Aldrich

Anti-phospho RasGRP2 (Ser116/Ser117)

from rabbit

Synonym(s):

RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, F25B3.3 kinase-like protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, monkey

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG

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manufacturer/tradename

G-BioSciences 786-619T

manufacturer/tradename

G-BioSciences 786-618T

manufacturer/tradename

G-BioSciences 786-615T

manufacturer/tradename

G-BioSciences 786-613T

pore size

50 K MWCO

pore size

15 K MWCO

pore size

1 K MWCO

pore size

15 K MWCO

General description

RAS guanyl-releasing protein 2 (UniProt: Q9QUG9; also known as Calcium and DAG-regulated guanine nucleotide exchange factor I, RasGRP2, CalDAG-GEFI, F25B3.3 kinase-like protein) is encoded by the Rasgrp2 gene (Gene ID: 19395) in murine species. RasGRP proteins are considered as activators of Ras and other related small GTPases by virtue o functioning as guanine nucleotide exchange factors (GEFs). RasGRP-1 through -4 share many structural domains, however, they display subtle differences between each of the different family members. They are expressed in distinct patterns, such as in different cells of the hematopoietic system and in the brain. RasGRP2′s expression is restricted to platelets and their precursors, megakaryocytes as well as neutrophils. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS, but not HRAS. It is also reported to functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils, probably through inside-out integrin activation. RaGRP2 is found both in the cytosol and associated with membranes. It is shown to be enriched at juxtamembrane areas and membrane ruffles. Three isoforms of RasGRP2 have been reported that are generated by alternative splicing. It contains two EF-hand domains, one N-terminal Ras-GEF domain, one phorbol-ester/DAG-type zinc finger domain, and one Ras-GEF domain. RasGRP2 deficient animals exhibit bleeding problems due to defects in platelets aggregation and degranulation. (Ref.: Olga Ksionda, O et al. (2013). Front Biol (Beijing). 8(5): 508 532).

Specificity

This rabbit polyclonal antibody detects RAS guanyl-releasing protein 2 phosphorylated on Ser116/117 in Mouse and Monkey cells. It targets an epitope with in 12 amino acids surrounding Ser116/Ser117 from the N-terminal region.

Immunogen

A linear peptide corresponding to 12 amino acids surrounding phosphorylated Ser116/117 from the N-terminal region of murine RAS guanyl-releasing protein 2.
Epitope: unknown

Application

Anti-phospho RasGRP2 (Ser116/Ser117), Cat. No. ABS2130, is a rabbit polyclonal antibody that detects RAS guanyl-releasing protein 2 phosphorylated on Ser116/Ser117 and has been tested for use in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A representative lot detected phospho RasGRP2 (Ser116/Ser117) in nucleus accumbens (NAc) from Drd1-mVenus transgenic mice treated with cocaine (Nagai, T., et. al. (2016). Neuron. 89(3):550-65).

Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser116/Ser117) in RasGRP2 purified from GST-Rasgrp2 transfected COS-7 cells and phosphorylated by GST-PKAc alpha, Forskolin stimulated mouse striatal muscle slices (Courtesy of Dr. Kozo Kaibuchi at Nagoya University, Japan).

Western Blotting Analysis: A representative lot detected phospho RasGRP2 (Ser116/Ser117) in a kinase assay with GST-Rasgrp2 (amino acids 1-374) and PKA, Forskolin and SKF81279 stimulated mouse striatal muscle slices, COS-7 cells transfected with EGFP-Rasgrp2 and Myc-PKA, nucleus accumbens (NAc) from mice treated with cocaine, Nucleus accu (Nagai, T., et. al. (2016). Neuron. 89(3):550-65).
Research Category
Signaling

Quality

Evaluated by Western Blotting in RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser116/Ser117 by GST-PKAc alpha.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RAS guanyl-releasing protein 2 in RasGRP2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser116/Ser117 by GST-PKAc alpha

Target description

~90 kDa observed; 69.45 kda calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 10% Ethylene Glycol, pH 7.6 with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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