ABE1339
Anti-dimethyl-BAF155 Antibody (Arg1064, asymetrically di-methylated)
from rabbit, purified by affinity chromatography
Synonym(s):
BRG1-associated factor 155
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
ChIP: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
dimethylation (Arg1064)
Gene Information
human ... SMARCC1(6599)
General description
BAF155, also known as SWI/SNF complex subunit BRG1-associated factor 155, BAF155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, and encoded by the gene name SMARCC1 and BAF155, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). BAF155 has been implied to stimulate the ATPase activity of the catalytic subunit of the complex. BAF155 may also be involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. BAF155 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). BAF155 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.
Immunogen
Epitope: C-terminus
linear peptide corresponding to the C-terminus of Human BAF155 protein dimethylated at Arg 1064.
Application
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Chromatin Biology
Chromatin Biology
This Anti-dimethyl-BAF155 antibody is validated for use in WB, IP, ChIP, IH for the detection of dimethyl-BAF155.
Western Blotting Analysis: A representative lot of this antibody detected methylated BAF155 in transfected MCF-7 cell lysate (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated methylated BAF155 from transfected SKOV3 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
ChIP: A representative lot of this antibody chromatin immunoprecipitated BAF155 to COL1A2 and GADD45A genes in wildtype MCF-7 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Immunohistochemical Analysis: A representative lot of this antibody detected methylated BAF155 protein in human breast tumor cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated methylated BAF155 from transfected SKOV3 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
ChIP: A representative lot of this antibody chromatin immunoprecipitated BAF155 to COL1A2 and GADD45A genes in wildtype MCF-7 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Immunohistochemical Analysis: A representative lot of this antibody detected methylated BAF155 protein in human breast tumor cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)
Quality
Evaluated by Western Blotting on MCF-7 cell lysate
Western Blotting Analysis: A 1:1000 dilution of this antibody detected dimethyl-BAF155 protein in 10 µg of MCF-7 cell lysate
Western Blotting Analysis: A 1:1000 dilution of this antibody detected dimethyl-BAF155 protein in 10 µg of MCF-7 cell lysate
Target description
~ 160 kDa observed. The ~160 kDa band has been confirmed to be the target protein BAF155 by mass spectrometry
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 10% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lu Wang et al.
Cancer cell, 25(1), 21-36 (2014-01-18)
Coactivator-associated arginine methyltransferase 1 (CARM1), a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which
Sergey Karakashev et al.
Nature communications, 9(1), 631-631 (2018-02-13)
CARM1 is an arginine methyltransferase that asymmetrically dimethylates protein substrates on arginine residues. CARM1 is often overexpressed in human cancers. However, clinically applicable cancer therapeutic strategies based on CARM1 expression remain to be explored. Here, we report that EZH2 inhibition
Sergey Karakashev et al.
Cancer cell, 37(2), 157-167 (2020-02-01)
In response to DNA double-strand breaks, MAD2L2-containing shieldin complex plays a critical role in the choice between homologous recombination (HR) and non-homologous end-joining (NHEJ)-mediated repair. Here we show that EZH2 inhibition upregulates MAD2L2 and sensitizes HR-proficient epithelial ovarian cancer (EOC)
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