AB5118
Anti-Cyclooxygenase-2 Antibody
Chemicon®, from rabbit
Synonym(s):
COX-2, PGHS-2, PHS-2
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
bovine, sheep, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PTGS2(5743)
Specificity
Cyclooxygenase-2. Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 and COX2. Both forms of COX proteins are membrane associated heme proteins containing cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin. COX2 is a 72 kDa protein having 60% homology to COX1. It is induced by cytokines and mitogens and likely plays a role in inflammatory diseases such as rheumatoid arthritis.
Immunogen
17 amino acid synthetic peptide corresponding to a sequence near the C-terminus of Human/Mouse/Rat COX-2 coupled to KLH.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neuroinflammation & Pain
This Anti-Cyclooxygenase-2 Antibody is validated for use in ELISA, WB for the detection of Cyclooxygenase-2.
Western blot: 1-10 μg/mL using Chemiluminescence technique; COX-2 runs as a 68-72KDa protein in most cells, sometimes a 90kDa post-translationally modified form is seen as well. CaCo-2 line extracts (induced with serum or growth factors) are good positive controls.
ELISA: 1:10,000 - 1:100,000 using 100 ng of immunogen peptide per well.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000 - 1:100,000 using 100 ng of immunogen peptide per well.
Optimal working dilutions must be determined by end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Clark A Lindgren et al.
The Journal of physiology, 591(19), 4749-4764 (2013-07-03)
Previous work has demonstrated that activation of muscarinic acetylcholine receptors at the lizard neuromuscular junction (NMJ) induces a biphasic modulation of evoked neurotransmitter release: an initial depression followed by a delayed enhancement. The depression is mediated by the release of
Gaetano Donofrio et al.
Reproduction (Cambridge, England), 134(1), 183-197 (2007-07-21)
Bovine postpartum uterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the gamma-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses
Qiong Cao et al.
PloS one, 12(10), e0186764-e0186764 (2017-10-20)
Microglia activation and associated inflammatory response are involved in the pathogenesis of different neurodegenerative diseases. We have reported that Notch-1 and NF-κB/p65 signalling pathways operate in synergy in regulating the production of proinflammatory mediators in activated microglia. In the latter
Beatriz Pérez-Cadahía et al.
The Journal of biological chemistry, 286(1), 42-49 (2010-11-13)
Activated by the RAS-MAPK signaling pathway, MSK1 is recruited to immediate-early gene (IEG) regulatory regions, where it phosphorylates histone H3 at Ser-10 or Ser-28. Chromatin remodelers and modifiers are then recruited by 14-3-3 proteins, readers of phosphoserine marks, leading to
Samriddhi Shukla et al.
Scientific reports, 6, 21860-21860 (2016-02-26)
Lack of effective anti-metastatic drugs creates a major hurdle for metastatic lung cancer therapy. For successful lung cancer treatment, there is a strong need of newer therapeutics with metastasis-inhibitory potential. In the present study, we determined the anti-metastatic and anti-angiogenic
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service