A-003-M
Poly-ᴅ-Lysine Hydrobromide
synthetic, liquid, 1 mg/mL, suitable for cell culture
Synonym(s):
poly lysine
About This Item
Recommended Products
product name
Poly-D-Lysine solution, 1.0 mg/mL, The Poly-D-Lysine solution promotes the adhesion of tissues/sections to the culture vessel.
Quality Level
form
liquid
manufacturer/tradename
Specialty Media
technique(s)
cell culture | mammalian: suitable
input
sample type neural stem cell(s)
sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
General description
Application
- To coat culture vessels and provide a suitable surface for the growth and attachment of neuronal cells.
- In preparing the surface of coverslips for facilitating the attachment of non-adherent cells.
Biochem/physiol Actions
Features and Benefits
- Poly-D-Lysine (PDL) solution is diluted in phosphate buffer saline (PBS), water, or 0.1M borate buffer.
- Working dilutions of 50μg/mL to 100μg/mL are usually adequate.
- It has a surface coverage of 3-10μg/cm2.
- PDL can be stored at -20 ˚C for up to 18 months.
- Plates are coated for 3 hours overnight and up to 3 days when stored at 4˚C.
- PDL-coated plates are used immediately or stored in PBS for up to 5 days.
Physical form
Preparation Note
Storage and Stability
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
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