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662065

Sigma-Aldrich

Ubiquitin, Methylated, Human, Recombinant

Synonym(s):

Me-Ub

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Pricing and availability is not currently available.

Assay

≥95% (SDS-PAGE)

Quality Level

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

solubility

aqueous buffer: 50 mg/mL

shipped in

wet ice

storage temp.

−20°C

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This Item
81077N81070581066N
assay

29-31 g/dL protein basis (biuret)

assay

29-31 g/dL protein basis (biuret)

assay

29-31 g/dL protein basis (biuret)

assay

≥98% (purity)

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

blood typing: suitable, enzyme immunoassay: suitable

technique(s)

enzyme immunoassay: suitable, microbiological culture: suitable, protein quantification: suitable

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

form

solution

form

solution

form

solution

form

lyophilized powder

packaging

pack of 10 L

packaging

pack of 50 mL

packaging

pack of 1 L

packaging

pack of 10 g

General description

Recombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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