17-10077
Magna ChIP® HT96 Chromatin Immunoprecipitation Kit
The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.41
Quality Level
manufacturer/tradename
Magna ChIP®
technique(s)
immunoprecipitation (IP): suitable
shipped in
dry ice
General description
The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument.Each kit includes a complete set of validated, quality controlled reagents, plus a detailed protocol.
Features & Benefits
Features & Benefits
- Complete set of materials for up to 96 ChIP reactions
- Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
- Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
- Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
- Protocols for using cells or tissues
- Direct analysis of DNA without additional clean-up steps
- Compatible with ChIPAb+ validated antibody and primer sets
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Used to detect/quantify: Protein A+G
Packaging
Kit capacity: 96 chromatin immunoprecipitation assays
Components
Magna ChIP protein A/G magnetic beads
HT96 Nuclei Isolation Buffer
HT96 ChIP Buffer (Sonication/ChIP/Wash)
Low Stringency IP Wash Buffer
HT96 ChIP Elution Buffer
Proteinase K Solution
Protease Inhibitor Cocktail III
10X Glycine
10X PBS
96 Well ChIP Plate
96 Well Thermal Plate
Plate Seal
Strip Caps
HT96 Nuclei Isolation Buffer
HT96 ChIP Buffer (Sonication/ChIP/Wash)
Low Stringency IP Wash Buffer
HT96 ChIP Elution Buffer
Proteinase K Solution
Protease Inhibitor Cocktail III
10X Glycine
10X PBS
96 Well ChIP Plate
96 Well Thermal Plate
Plate Seal
Strip Caps
Physical form
Two boxes containing all necessary reagents to perform 96 chromatin immunoprecipitation reactions in a 96 well plate format. Supplied buffers are sufficient to generate 24 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.
Storage and Stability
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Legal Information
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10-13 - German Storage Class 10 to 13
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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