07-2119

Anti-phospho-RalA Antibody (Ser194)

from rabbit, purified by affinity chromatography

• Synonym(s): Ras-related protein Ral-A
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• technique(s): inhibition assay: suitable (peptide); western blot: suitable
• NCBI accession no.: NP_005393
• UniProt accession no.: P11233
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer194)
• Gene Information: human ... RALA(5898)

Description

General description

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.

Specificity

This antibody recognizes RalA phosphorylated at Ser194.

Immunogen

KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.

Application

Detect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.

Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.

Quality

Evaluated by Western Blot in PANC10.05 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.

Target description

~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.

Analysis Note

Control
PANC10.05 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable