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Key Documents

05-753

Sigma-Aldrich

Anti-WT1 Antibody, clone 6F-H2

clone 6F-H2, Upstate®, from mouse

Synonym(s):

Anti-AWT1, Anti-GUD, Anti-NPHS4, Anti-WAGR, Anti-WIT-2, Anti-WT-1, Anti-WT33

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6F-H2, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_024426

UniProt accession no.

P19544

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... WT1(7490)

Specificity

WT1

Immunogen

6His-tagged fusion protein corresponding to residues 1-181 of human WT1 (Wilms tumor)

Application

Anti-WT1 Antibody, clone 6F-H2 is a Mouse Monoclonal Antibody for detection of WT1 also known as Wilms′ Tumor & has been tested in WB, ICC & IHC.

Quality

routinely evaluated by immunoblot on RIPA lysates from Jurkat and Raji cells

Target description

55-60kDa

Physical form

Format: Purified

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

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Toru Sakairi et al.
American journal of physiology. Renal physiology, 298(3), F557-F567 (2009-12-04)
Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture conditions. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term
Zenglai Tan et al.
Scientific reports, 8(1), 16618-16618 (2018-11-11)
The kidney is a complex organ that is comprised of thousands of nephrons developing through reciprocal inductive interactions between metanephric mesenchyme (MM) and ureteric bud (UB). The MM undergoes mesenchymal to epithelial transition (MET) in response to the signaling from
Toru Sakairi et al.
American journal of physiology. Renal physiology, 299(4), F802-F809 (2010-07-30)
We transformed mouse podocytes by ectopic expression of cyclin-dependent kinase 4 (CDK4). Compared with podocytes transformed with a thermo-sensitive SV40 large T antigen mutant tsA58U19 (tsT podocytes), podocytes transformed with CDK4 (CDK4 podocytes) exhibited significantly higher expression of nephrin mRNA.
Rosalba Parenti et al.
PloS one, 9(12), e114333-e114333 (2014-12-05)
Wilms' tumor gene 1 (WT1) plays complex roles in tumorigenesis, acting as tumor suppressor gene or an oncogene depending on the cellular context. WT1 expression has been variably reported in both benign and malignant peripheral nerve sheath tumors (MPNSTs) by
Aleksandra Rak-Raszewska et al.
Developmental dynamics : an official publication of the American Association of Anatomists (2021-09-09)
Tissue organoids derived from primary cells have high potential for studying organ development and diseases in numerous organs. They recreate the morphological structure and mimic the functions of given organ while being compact in size, easy to produce, and suitable
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