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Key Documents

05-372

Sigma-Aldrich

Anti-GRB2 Antibody, clone 3F2

clone 3F2, Upstate®, from mouse

Synonym(s):

Anti-ASH, Anti-EGFRBP-GRB2, Anti-Grb3-3, Anti-MST084, Anti-MSTP084, Anti-NCKAP2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

3F2, monoclonal

species reactivity

bovine, mouse, hamster, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_002086

UniProt accession no.

P62993

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GRB2(2885)

Specificity

N-terminal SH3 region of GRB2

Immunogen

Recombinant GRB2/Ash

Application

This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.

Quality

routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract

Target description

23-25kDa

Physical form

Format: Purified
PBS containing 50% glycerol

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rucci, N; DiGiacinto, C; Orru, L; Millimaggi, D; Baron, R; Teti, A
Journal of Cell Science null
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
Stein-Gerlach, M., et al
The Journal of Biological Chemistry, 270, 24635-24637 (1995)
M Rozakis-Adcock et al.
Nature, 363(6424), 83-85 (1993-05-06)
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Y Chen et al.
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