04-642

Anti-Ago2 Antibody, clone 9E8.2

ascites fluid, clone 9E8.2, Upstate^®

• Synonym(s): Argonaute-2, Eukaryotic translation initiation factor 2C, AGO2, eIF-2C, Slicer protein, MGC3183, Piwi domain protein, PPD
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 9E8.2, monoclonal
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_012154
• UniProt accession no.: Q9UKV8
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... AGO2(27161)

Description

General description

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

Specificity

human Ago2

Immunogen

Epitope: near the N-terminus of Ago2

KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG

Application

Anti-Ago2 Antibody, clone 9E8.2 is a Mouse Monoclonal Antibody for detection of Ago2 also known as Argonaute-2, Eukaryotic translation initiation factor 2C & has been validated in WB and has been demonstrated to perform in ChIP and ChIP-seq.

ChIP and ChIP-seq: A representative lot of this antibody was used to perform ChIP and ChIP-seq ( Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Chromatin Biology

RNA Binding Protein (RBP)

Quality

Routinely evaluated by immunoblot.

Target description

~100 kda

Physical form

Ascites with 0.05% sodium azide

ImmunoAffinity Purified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable