803316
Sulfo-LC-SPDP (sulfosuccinimidyl 6-[3′-(2-pyridyldithio)propionamido]hexanoate)
Synonym(s):
2,5-Dioxo-3-sulfo-1-pyrrolidinyl 6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]hexanoate, Sulfo-LC SPDP, Sulfosuccinimidyl 6-(3′-(2-pyridyldithio)propionamido)hexanoate
About This Item
Recommended Products
Assay
≥90%
Quality Level
form
powder
mol wt
527.57
reaction suitability
reagent type: cross-linking reagent
storage condition
desiccated
solubility
soluble (>95 mg/ml in water)
shipped in
ambient
storage temp.
−20°C
SMILES string
O=C1CC(S(=O)([O-])=O)C(N1OC(CCCCCNC(CCSSC2=CC=CC=N2)=O)=O)=O.[Na+]
InChI
1S/C18H23N3O8S3.Na/c22-14(8-11-30-31-15-6-3-5-10-20-15)19-9-4-1-2-7-17(24)29-21-16(23)12-13(18(21)25)32(26,27)28;/h3,5-6,10,13H,1-2,4,7-9,11-12H2,(H,19,22)(H,26,27,28);/q;+1/p-1
InChI key
ZAPNXDUFCQIHFS-UHFFFAOYSA-M
General description
Features and Benefits
- Reactive groups: NHS ester and pyridyldithiol
- Reactive towards: amino and sulfhydryl groups
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
- Cleavable crosslinker allows separation of crosslinked products
- Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
- Compare to other varieties of SPDP-type reagents, including pegylated forms
Caution
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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