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368881

Sigma-Aldrich

Fast Red TR Salt

Dye content 15 %

Synonym(s):

Fast Red TR Salt hemi(zinc chloride) salt, 4-Chloro-2-methylbenzenediazonium salt, Azoic Diazo No. 11

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About This Item

Empirical Formula (Hill Notation):
C7H6Cl2N2 · 0.5 ZnCl2
CAS Number:
Molecular Weight:
257.19
Colour Index Number:
37085
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

powder

composition

Dye content, 15%

λmax

284 nm

ε (extinction coefficient)

≥3000 at 281-287 nm in water at 0.006 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc(Cl)ccc1[N+]#N.Cc2cc(Cl)ccc2[N+]#N

InChI

1S/2C7H6ClN2.4ClH.Zn/c2*1-5-4-6(8)2-3-7(5)10-9;;;;;/h2*2-4H,1H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

RIIVFDKBXXMYRL-UHFFFAOYSA-J

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General description

Fast Red TR is a diazonium dye. It serves as a chromogenic substrate for alkaline phosphatase (AP), a common antibody conjugate widely used fin immunohistochemical applications.

Application

Fast Red TR Salt hemi (zinc chloride) salt has been used in the preparation of staining solutions to stain TRACP (tartrate-resistant acid phosphatase). It is also used for the detection of alkaline phosphatase.

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Embryonic alkaline phosphatase is expressed at M-phase in the spermatogenic lineage of the mouse
Narisawa S
Development, 116(1), 159-165 (1992)
Kessler C
Nonradioactive Labeling and Detection of Biomolecules (2012)
An improved in situ hybridization method for the detection of fish pathogens.
McCarthy UM, Urquhart KL, and Bricknell IR
Journal of Fish Diseases, 31(9), 669-677 (2008)
Reliability of new poly (lactic-co-glycolic acid) membranes treated with oxygen plasma plus silicon dioxide layers for pre-prosthetic guided bone regeneration processes
Castillo-Dali G
Medicina Oral, patologia Oral y cirugia Bucal, 22(2), e242-e250 (2017)
G E Hoffmann et al.
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie, 23(11), 759-763 (1985-11-01)
In the present study, the reaction of 15 different diazonium salts with serum bilirubin is examined. It is shown that the selectivity of the determination of non-conjugated and total bilirubin greatly depends on two conditions: the pH-value of the reaction

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