138630

1-Chloro-2,4-dinitrobenzene

97%

• Synonym(s): 2,4-Dinitrochlorobenzene, CDNB, DNCB
• Linear Formula: ClC₆H₃(NO₂)₂
• CAS Number: 97-00-7
• Molecular Weight: 202.55
• Beilstein: 613161
• EC Number: 202-551-4
• MDL number: MFCD00007075
• UNSPSC Code: 12352100
• PubChem Substance ID: 24848362
• NACRES: NA.22

Properties

• Quality Level: 100
• Assay: 97%
• form: solid
• expl. lim.: 22 %
• bp: 315 °C (lit.)
• mp: 48-50 °C (lit.)
• solubility: alcohol: soluble (hot); alcohol: very slightly soluble (cold); benzene: soluble; carbon disulfide: soluble; diethyl ether: soluble; water: insoluble
• functional group: chloro; nitro
• SMILES string: [O-][N+](=O)c1ccc(Cl)c(c1)[N+]([O-])=O
• InChI: 1S/C6H3ClN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H
• InChI key: VYZAHLCBVHPDDF-UHFFFAOYSA-N

Description

Application

1-Chloro-2,4-dinitrobenzene was used in determination of relative amounts of individual glutathione S-transferases subunits in human tissues by reverse-phase HPLC. It was used as contact sensitizer to study the antibacterial peptides hBD-2, -3 and -4 and LL-37 induced IL-18 protein release in human keratinocytes.

Biochem/physiol Actions

2,4-dinitrochlorobenzene induces atopic dermatitis in BALB/c mice model.

Preparation Note

The product is dissolved in ethanol when used as glutathione S-transferase substrate and then added to a buffer to form a final concentration of 1mM in phosphate buffer (8 mL of 0.2 M KH₂PO₄ and 28 mL of 0.1 M Na₂HPO₄ per 100 mL of solution, adjusted to pH 6.5) and 4% (v/v) ethanol.

Safety Information

• Pictograms: GHS06,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H310,H315,H317,H318,H410
• Precautionary Statements: P262 - P273 - P280 - P301 + P312 - P302 + P352 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point(F): 381.2 °F - closed cup
• Flash Point(C): 194 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves