Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Kluczowe dokumenty

808641

TSKgel® Ether-5PW (10 µm) HPLC Columns

L x I.D. 7.5 cm × 7.5 mm, ether phase

Synonim(y):

TSKgel® Ether-5PW HPLC Column

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
41115700
eCl@ss:
32110501

Nazwa produktu

TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size

Materiały

stainless steel

linia produktu

TSKgel®

producent / nazwa handlowa

Tosoh 08641

Parametry

≤4 M salt concentration
≤50% organic solvent
1.2 mL/min flow rate
10-50 °C temperature
20 bar max. pressure

metody

HPLC: suitable

dł. × śr. wewn.

7.5 cm × 7.5 mm

Matryca

polymer particle platform

grupa aktywna macierzy

ether phase

wielkość cząstki

10 μm

wielkość porów

1000 Å

pH robocze

2-12

metoda separacji

hydrophobic interaction (HIC)

Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów

Opis ogólny

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.TSKgel Ether-5PW, Phenyl-5PW, and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a very hydrophobic packing (e.g., phenyl in this case). We recommend an Ether-5PW column for purifying hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing rapid analyses. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

Postać fizyczna

Shipped in water.

Polecane produkty

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informacje prawne

TSKgel is a registered trademark of Tosoh Corporation
Ta strona może zawierać tekst przetłumaczony maszynowo.

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej