MABN630

Anti-SDHA Antibody, clone 7H11.2

clone 7H11.2, from mouse

• Synonim(y): Flavoprotein subunit of complex II, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 7H11.2, monoclonal
• reaktywność gatunkowa: human, mouse
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_004159
• numer dostępu UniProt: P31040
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... SDHA(6389)

Opis

Opis ogólny

SDHA, also known as Succinate dehydrogenase flavoprotein-mitochondrial subunit, or Flavoprotein subunit of complex II (Fp), and encoded by the gene symbol SDHA/ SDH2/ SDHF, is a member of the FAD-dependent oxidoreductase 2 family and the FRD/SDH subfamily. Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) is involved in the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Recent studies have indicated that SDHA may act as a tumor suppressor. SDHA is composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. SDHA interacts with SDHAF2/SDH5 in the process required for FAD attachment. Additionally, FDHA has been shown to interact with TRAP1. Mutations in the SDHA gene have been associated with Mitochondrial complex II deficiency (MT-C2D), a disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Additionally, mutations in the SDHA gene have been implied in Leigh syndrome or Kearns-Sayre syndrome, Cardiomyopathy dilated 1GG (CMD1GG), and Paragangliomas 5 (PGL5).

Immunogen

Epitope: Immunogen is near the C-terminal.

GST-tagged recombinant protein corresponding to the C-terminus of Human SDHA.

Zastosowanie

Anti-SDHA, clone 7H11.2 is an antibody against SDHA for use in WB, IH.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western Blotting Analysis: A 0.5 µg/mL dilution from a representative lot detected SDHA in 10 µg of C2C12 and HeLa cell lysates.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SDHA in human cerebral cortex and human cardiac myocytes tissues.

Jakość

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 0.5 µg/mL diultion of this antibody detected SDHA in 10 µg of Human brain tissue lysate.

Opis wartości docelowych

~68 kDa observed

Postać fizyczna

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable