Skip to Content
Merck
All Photos(1)

Key Documents

G005858

LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: 5 mM ammonium formate in methanol:water (75:25)
flow rate: 0.4 mL/min
column temp.: 40 °C
detector: UV, 265 nm (MS, ESI(+), m/z 100-1000)
injection: 1 μL
sample: 20 μg/mL in methanol

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Analysis Note

The 25-OH forms of the secosteroids vitamin D2 and D3 are indicators of the vitamin D status of an individual. However, because they have different biological activity and clinical interest, they should be distinguishable. The Ascentis Express F5 phase was able to resolve the two compounds under highly LC-MS/MS compatible conditions. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    25-Hydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
    25-Hydroxyvitamin D2 solution, 50 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service