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G003725

HPLC Analysis of Ribonucleosides on Ascentis® Si

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)
mobile phase: [A] 150 mM ammonium formate (pH 3.0, with formic acid): [B] acetonitrile; (10:90, A:B)
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: UV, 270 nm
injection: 1 μL
sample: as indicated in mobile phase

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Uridine, ≥99%
    Adenosine, ≥99% .1 mg/mL
    Guanosine, ≥98% .1 mg/mL
    Cytidine, ≥99.0% (HPLC)

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