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TSKgel® UltraSW Aggregate (3 µm) HPLC Columns

L x I.D. 30 cm × 7.8 mm, diol phase

Synonym(s):

TSKgel® Size Exclusion (SW-Type) HPLC Column

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About This Item

UNSPSC Code:
41115711

product name

TSKgel® UltraSW Aggregate HPLC Column, phase diol, L × I.D. 30 cm × 7.8 mm, 3 μm particle size

material

stainless steel

Agency

suitable for USP L20
suitable for USP L59

product line

TSKgel®

manufacturer/tradename

Tosoh UltraSW Aggregate, 22856

parameter

≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

30 cm × 7.8 mm

matrix

silica particle platform

matrix active group

diol phase

particle size

3 μm

pore size

300 Å

operating pH

2.5-7.5

separation technique

size exclusion (SEC)

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General description

The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best for aggregation, dimer, and fragmentation, making it the best method for heterogeneity studies.

TSKgel UltraSW Aggregate columns have a slightly larger pore size and smaller (3 μm) particle size than the industry-leading TSKgel G3000SWxl (5 μm) and SuperSW3000 (4 μm) columns. These 30 cm x 7.8 mm ID silica-based, stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.

The calibration curve of the TSKgel UltraSW Aggregrate column shows a separation range up to around 2 million Da, which provides better resolution of aggregates/multimers of a mAb than the TSKgel SuperSW mAb columns..

Physical form

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Articles

Columns and Analytical Standards for Protein SEC

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

Protocols

Calibration Curves for TSKgel® SW mAb Columns

Calibration Curves for TSKgel® SW mAb Columns

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