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50347-U

Supelco

Ascentis® Express OH5, 5 μm HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

stainless steel column

Quality Level

100

Agency

suitable for USP L86

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

90 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

hydroxy, penta- phase

particle size

5 μm

pore size

90 Å pore size

operating pH range

2-9(lit.)

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)

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General description

Ascentis Express 5 μm OH5 is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell and an overall particle size of ~5-microns. The Ascentis Express 5 μm OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. This high performance material provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USHYU001306
USHYU001305
USHYU001304
USHYU001297
USHYU001295

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Paweł Kubica et al.
Analytical and bioanalytical chemistry, 406(13), 3013-3018 (2014-03-26)
The influence of sucrose combustion products on smoking and nicotine addiction is still controversial because the presence of the sucrose may be treated as a source of aldehydes and organic acids. In e-liquids used as refills for electronic cigarettes, which

Articles

USP HPLC Analysis of L-Methionine on Ascentis® Express OH5

Separation of L-Methionine, Pharmaceutical Secondary Standard; Certified Reference Material

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