ZRB1560
Anti-eIF-2α Antibody, clone 3M6 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Eukaryotic translation initiation factor 2 subunit 1
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3M6, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 36.11 kDa
observed mol wt ~36 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... EIF2S1(1965)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3M6 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 2a (eIF-2a).
Immunogen
His-tagged recombinant full-length human Eukaryotic translation initiation factor 2 subunit a (eIF-2a )
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected eIF-2a in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in Raji cell lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in HeLa cell line, A431, HUVEC, and NIH 3T3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound eIF-2a with a KD of 2.6 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected eIF-2a in one million Ramos cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected eIF-2a in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in Raji cell lysate.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF-2a in HeLa cell line, A431, HUVEC, and NIH 3T3 cells.
Affinity Binding Assay:: A representative lot of this antibody bound eIF-2a with a KD of 2.6 x 10-8 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected eIF-2a in one million Ramos cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Eukaryotic translation initiation factor 2 subunit 1 (UniProt: P05198; also known as Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha) is encoded by the EIF2S1 (also known as EIF2A) gene (Gene ID: 1965) in human. eIF-2A is a heterotrimer composed of a, b, and g chains. It functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Its activity is regulated by phosphorylation at serine 48 and serine 51 (without methionine), which stabilizes the eIF-2/GDP/eIF-2B complex and prevents the GDP/GTP exchange reaction, thus impairing the recycling of eIF-2a between successive rounds of initiation and leading to global inhibition of translation. elF-2a is reported to be a substrate for eukaryotic translation initiation factor 2-alpha kinase 1 to 4 (EIF2AK1, 2, 3, and 4). It is also a key component of the integrated stress response (ISR) and is required for adaptation to various stress conditions. Phosphorylation on serine 51 by the EIF2AK4 is shown to occur in response to amino acid starvation and UV irradiation. Its phosphorylation by EIF2AK1-4 converts it into a global protein synthesis inhibitor, leading to reduction in cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, YY., et al. (2009). J. Biol. Chem. 284(11); 6661-6673; Mikami, S., et al. (2006). Protein Expr, Purif. 46(2); 348-357).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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