X0879

Anti-XPB antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-BTF2, Anti-ERCC3, Anti-Excision repair cross-complementing rodent repair deficiency, complementation group 3
• MDL number: MFCD02263308
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 90 kDa
• species reactivity: human, rat, mouse
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 10-20 μg/mL using HeLa cells fixed with paraformaldehyde-Triton; western blot: 1-2 μg/mL using HepG2 cell lysates
• UniProt accession no.: P19447
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ERCC3(2071) ; mouse ... Ercc3(13872) ; rat ... Ercc3(291703)

Description

General description

XPB (Xeroderma pigmentosum complementation group B) is located on the human chromosome at 2q14.3. TFIIH (General transcription and DNA repair factor IIH helicase) consists of nine protein subunits, XPB (ERCC3) being one of them. It is an ATP-dependent DNA helicase of 89 kDa. The XPB is localized in the neurons.

Immunogen

synthetic peptide corresponding to amino acids 765-782 of human XPB, also known as ERCC3, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences in one and three amino acids respectively.

Application

Anti-XPB antibody has been used in immunoblotting andimmunofluorescence.

Biochem/physiol Actions

XPB (General transcription and DNA repair factor IIH helicase subunit XPB) is involved in DNA repair and transcription. Phosphorylation on Ser⁷⁵¹ of XPB inhibits TFIIH repair activity, leaving its transcription function intact. Mutations in the gene encoding XPB causes Xeroderma Pigmentosum, Cockayne′s syndrome, and trichothiodystrophy.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves