T3449
TrypZean® Solution, 1×
recombinant, expressed in corn, sterile-filtered
Synonym(s):
Trypsin
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About This Item
Recommended Products
recombinant
expressed in corn
Quality Level
sterility
sterile-filtered
form
aqueous solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable
pH
7.70-8.30
shipped in
dry ice
storage temp.
−20°C
General description
TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.
Application
TrypZean® Solution, 1× has been used:
- for the suspension of pellet during DNA extraction
- digestion of cells to single-cell suspension for pharmacotyping of 2D cells
- as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium
Features and Benefits
TrypZean® Solution provides the following features and benefits:
- Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
- TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
- Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
- High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
- Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines
Legal Information
Trypzean is a registered trademark of ProdiGene, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
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