SRP0210

PRL-3 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): PRL-3, PTP4A3, Protein tyrosine phosphatase type IVA, member 3, Protein-tyrosine phosphatase of regenerating liver 3
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 19.5 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_032611
• UniProt accession no.: O75365
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PTP4A3(11156)

Description

General description

Human PRL 3 (GenBank Accession No. NM_032611), (amino acids 2-173) with N-terminal HIS tag, MW = 19.5 kDa, expressed in an E Coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.