SIALICQ
Sialic Acid Quantitation Kit
Sufficient for 25 reactions
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About This Item
UNSPSC Code:
41116107
NACRES:
NA.32
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shipped in
wet ice
storage temp.
2-8°C
General description
The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total N-acetylneuraminic acid (NANA; sialic acid). NANA is released from glycoconjugates using neuraminidase. The kit uses α(2→3,6,8,9) neuraminidase to cleave all NANA linkages, including α(2→8) and α(2→9) linkages, as well as branched NANA, for the most accurate determination of total sialic acid content.
Application
Contains all reagents to quantitate the amount of N-acetylneuraminic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids and capsular polysaccharides (consisting only of polysialic acid).
Quantity
Sufficient reagents are provided to perform 25 reactions, including control samples. Each reaction will measure 1-200 nmoles of NANA.
Kit Components Only
Product No.
Description
- N-Acetylneuraminic acid aldolase 25 μL
- N-Acetylneuraminic acid 200 μL
- α(2→3,6,8,9)-Neuraminidase 25 μL
- Fetuin, bovine .5 mg
- Lactic dehydrogenase 25 μL
- β-NADH disodium salt 3 vial(s)
- Tris-HCl, pH 7.5 1 mL
- Sialidase buffer 500 μL
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
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