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SH1321

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

Cytokine and Chemokine Receptors

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

Pricing and availability is not currently available.

product line

MISSION®

storage temp.

−20°C

Gene Information

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WHA1001824WHA10347893WHA1001932
manufacturer/tradename

Whatman 10347890, Whatman Article No. 28414667 (US reference)

manufacturer/tradename

Whatman 1001-824, Whatman Article No. 28413935 (US reference)

manufacturer/tradename

Whatman 10347893, Whatman Article No. 28414668 (US reference)

manufacturer/tradename

Whatman 1001-932, Whatman Article No. 28413942 (US reference)

W × L

150 mm × 150 mm

W × L

75 mm × 100 mm

W × L

100 mm × 100 mm

W × L

580 mm × 680 mm

packaging

pkg of 500 ea

packaging

pack of 500

packaging

pkg of 500 ea

packaging

pack of 500

feature

Grade 2122

feature

Grade 1

feature

Grade 2122

feature

Grade 1

basis weight

40 g/m2

basis weight

87 g/m2

basis weight

40 g/m2

basis weight

87 g/m2

General description

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 93, Number of Clones: 584
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Céline Callewaere et al.
Journal of molecular endocrinology, 38(3), 355-363 (2007-03-07)
Chemokines are small secreted proteins that chemoattract and activate immune and non-immune cells both in vivo and in vitro. In addition to their well-established role in the immune system, several recent reports have suggested that chemokines and their receptors may
Samantha J Allen et al.
Annual review of immunology, 25, 787-820 (2007-02-13)
Chemokines are critical mediators of cell migration during routine immune surveillance, inflammation, and development. Chemokines bind to G protein-coupled receptors and cause conformational changes that trigger intracellular signaling pathways involved in cell movement and activation. Although chemokines evolved to benefit

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