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Key Documents

SAB4301134

Sigma-Aldrich

Anti-6-His antibody produced in rabbit

affinity isolated antibody

Synonym(s):

6 His epitope tag, Anti His Antibody Sigma, Anti His Antibody Sigma - Anti-6×His antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

46 kDa

concentration

0.5 mg/mL

technique(s)

western blot: 1:500-1:2000 (Cell Lysate)

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Specificity

The antibody detects transfected proteins contanining 6×His tag.

Immunogen

Synthetic peptide: 18XHis conjugated to KLH

Application

Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
The Anti-6-His antibody has been used in western blotting.

Biochem/physiol Actions

The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer.
Chen X, et al.
Theranostics, 8(9), 2348-2348 (2018)
O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency
Wang T, et al.
Nature Cell Biology, 19(7), 833-833 (2017)
The FLAG? peptide, a versatile fusion tag for the purification of recombinant proteins.
Einhauer A and Jungbauer A
Journal of Biochemical and Biophysical Methods, 49(1-3), 455-465 (2001)
Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation
Jiang Y, et al.
Nature Cell Biology, 17(9), 1158-1158 (2015)
Ningning Shi et al.
Advanced biology, 6(11), e2200092-e2200092 (2022-07-13)
Site-specific incorporation of distinct noncanonical amino acids (ncAAs) into proteins via genetic code expansion in mammalian cells represents a new avenue for protein engineering. Reassigning three TAGs with the same ncAA in mammalian cells has previously been achieved using translational
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