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Key Documents

R0656

Sigma-Aldrich

Anti-Rab14 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-F protein-binding protein 1, Anti-GTPase Rab14, Anti-RAB14, member RAS oncogene family, Anti-Small GTP binding protein RAB14

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~24 kDa

species reactivity

mouse, human, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using whole extract of human Raji cells
indirect immunofluorescence: 2.5-5 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 1-2 μg/mL using whole extract of mouse brain

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB14(51552)
mouse ... Rab14(68365)
rat ... Rab14(94197)

Related Categories

General description

Rab14 (Ras-related protein) is a Golgi-related Rab, that is located on chromosome 9q33.2. It is present in the endo-lysosomal pathway and contractile vacuole system of membranes. It is expressed almost in all tissues.
Rab14 (Ras-related protein) is a member of the Rab family of small guanosine triphosphatases (GTPases).

Application

Anti-Rab14 (C-terminal) antibody produced in rabbit has been used in:immunoblotting, immunoprecipitation, indirect immunofluorescence.

Biochem/physiol Actions

Rab14 (Ras-related protein) participates in membrane trafficking between the Golgi complex and endosomes. It may play an important role in surfactant secretion.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The functions of Rab GTPases in plant membrane traffic
Woollard AAD and Moore I
Current Opinion in Plant Biology, 11(6), 610-619 (2008)
Annexin A2 interactions with Rab14 in alveolar type II cells
Gou D, et al.
The Journal of Biological Chemistry, 283(19), 13156-13164 (2008)
RabD, a Dictyostelium Rab14-related GTPase, regulates phagocytosis and homotypic phagosome and lysosome fusion
Harris E and Cardelli J
Journal of Cell Science, 115(Pt 18), 3703-3713 (2002)
A large-scale rheumatoid arthritis genetic study identifies association at chromosome 9q33.2
Chang M, et al.
PLoS Genetics, 4(6) (2008)
A Lacoma et al.
Virulence, 8(8), 1761-1775 (2017-08-02)
Staphylococcus aureus is a particularly difficult pathogen to eradicate from the respiratory tract. Previous studies have highlighted the intracellular capacity of S.aureus in several phagocytic and non-phagocytic cells. The aim of this study was to define S.aureus interaction within a

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