P9582
Polyinosinic–polycytidylic acid potassium salt
with buffer salts, TLR ligand tested
Synonym(s):
Poly (I:C), Poly(I) • Poly(C)
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About This Item
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Quality Level
Assay
≥99% (less than 1% free nucleotides, TLC)
form
lyophilized powder
composition
Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)
storage condition
desiccated
storage temp.
−20°C
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Application
TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.
Biochem/physiol Actions
Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.
Packaging
Package size based on polynucleotide content
Other Notes
Double-stranded homopolymer.
Preparation Note
The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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