P350-05

Porcine Coronary Artery Smooth Muscle Cells: PCASMC (Cryovial)

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: Porcine coronary artery
• Quality Level: 100
• form: solid
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 38
• morphology: smooth muscle
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): diabetes; stroke; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

vascular research, supply of blood to heart muscle

Components

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PCASMC Culture Protocol.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable