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P2192

Sigma-Aldrich

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

Synonym(s):

PCR Buffer Solution, PCR Reaction Buffer

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About This Item

UNSPSC Code:
41106306
NACRES:
NA.52

Quality Level

300

form

liquid

packaging

vial of 1.5 mL

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

Application

10X PCR Buffer has been used as a component of the PCR mixture:
  • for the amplification of viral DNA
  • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
  • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Features and Benefits

  • Standalone buffer
  • Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Components

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
G J Breedveld et al.
Neurology, 59(4), 579-584 (2002-08-28)
Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
Cem Gokcen et al.
International journal of medical sciences, 8(7), 523-528 (2011-09-08)
The purpose of this study was to evaluate the relationship between 5,10- methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Attention Deficit Hyperactivity Disorder (ADHD) in a sample of Turkish children. MTHFR gene polymorphisms were assessed in 40 patients with ADHD and 30
Zhong-Dong Shi et al.
Stem cells translational medicine, 9(11), 1378-1388 (2020-07-15)
Human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) and embryonic stem cells, hold great promise for cell-based therapies, but safety concerns that complicate consideration for routine clinical use remain. Installing a "safety switch" based on the inducible caspase-9
View All Related Papers

Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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