M7383

Malic Dehydrogenase from porcine heart

≥400 units/mg protein (biuret), ammonium sulfate suspension

• Synonym(s): L-Malate: NAD⁺ oxidoreductase, MDH, Malate Dehydrogenase
• Enzyme Commission number: 1.1.1.37 (BRENDA, IUBMB)
• EC Number: 232-622-5
• MDL number: MFCD00131567
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: ammonium sulfate suspension
• specific activity: ≥400 units/mg protein (biuret)
• foreign activity: Glutamic-Oxalacetic Transaminase ≤0.01%; Glutamic-Pyruvic Transaminase ≤0.01%
• storage temp.: 2-8°C

Description

General description

Cytoplasmic Isozyme

Application

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.

Biochem/physiol Actions

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 0.1 M KH₂PO₄, pH 7.0

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves