M0644
Minimum Essential Medium Eagle
Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
MEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
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General description
Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.
Application
Minimum essential medium Eagle has been used to:
- maintain natural killer (NK) cell lines
- culture Leishmania donovani strains
- culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles
Quantity
Formulated to contain 10.1 grams of powder per liter of medium.
Reconstitution
Supplement with 2.2 g/L sodium bicarbonate.
also commonly purchased with this product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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