Name: Lentiviral Pools
Brand: SIGMA

CRISPR Lentrivirus<br>Order - GeCKOv2 Pool

<h2>Create Your Own L<span class="h2-lowercase">enti</span>ORF Pool</h2>


<h2>Create Your Own <span class="h2-lowercase">sh</span>RNA Pool</h2>


<h2>Knockout Genes using our CRISPR Pooled Lentivirus</h2>
<h3>CRISPR Whole Genome Pools</h3>


<h2>Knockdown Genes using our <span class="h2-lowercase">sh</span>RNA Pooled Lentivirus</h2>
<h3>shRNA Whole Genome Pools</h3>


<h2><span class="h2-lowercase">sh</span>RNA Human Kinase Pools</h2>


<h2>Overexpress Genes using our L<span class="h2-lowercase">enti</span>ORF Pooled Lentivirus</h2>
<h3>Pooled Human LentiORF Libraries</h3>


<h3>Whole genome CRISPR knockout screening for every lab</h3>
<ul>
<li>Utilize the power of CRISPR to knockout and screen every gene in the human genome</li>
<li>Select from the GeCKOv2 or our Whole Human Genome CRISPR Pools</li>
<li>Average of 6 gRNAs per gene (GeCKOv2); 10 gRNAs per gene</li>
<li>Divided into 2 subpools at 62,000 clones per pool (GeCKOv2, 124,000 clones total);8 subpools at 23,000 clones per pool (184,000 clones total)</li>
<li>Numerous built-in enrichment, depletion, and non-targeting control clones allow researchers to confidently gauge the success of their pooled screening experiments</li>
<li>2 vector system (pools are gRNA-only, Cas9 sold separately)</li>
<li>All pools are deep sequenced to verify clone representation and distribution</li>
<li>Deliverables: 200 μl of each pool at a minimum of 5x108 viral particles/ml (via p24), ships immediately</li>
</ul>


<h4>Create Your Own CRISPR Pool - Focus on the genes essential to your research</h4>
<ul>
<li>Special promotion: 10,000 pre-designed clones for $12,000</li>
<li>Custom viral titers, volumes, and aliquoting are available (standard deliverable: 200 μl at a minimum of 5 x 10<sup>8</sup> viral particles/ml in 4 x 50 μl aliquots); quotes available upon request</li>
<li>All pools are deep sequenced to verify clone representation and distribution</li>
<li>TAT: 8-10 weeks from finalization of clone list</li>
<li>DNA format also available</li>
</ul>


<h4>Focus on the genes essential to your research by creating your own custom shRNA pool</h4>
<ul>
<li>Create a custom shRNA pool to specifically target your genes of interest</li>
<li>Customize your viral titers, volumes, and aliquoting needs for greater flexibility in your screening experiments. DNA format also available.</li>
<li>Titers and volumes adequate for <i>in vitro</i>, <i>in vivo</i> and xenograft applications</li>
<li>Partner with our next-generation sequencing services for data deconvolution to get the most out of your assays</li>
</ul>


<h4>Maximize your overexpression screening efficiency by creating your own custom LentiORF pool</h4>
<ul>
<li>Create your custom LentiORF pool for maximum return of your overexpression screening</li>
<li>Customize your volume and aliquoting needs to further enhance your ability to rapidly screen multiple cell lines. DNA format also available</li>
<li>Titers and volumes adequate for <i>in vitro</i>, <i>in vivo</i> and xenograft applications</li>
<li>Partner with our next-generation sequencing services for data deconvolution to get the most out of your assays</li>
</ul>


<h4>Whole genome overexpression ORF screening at a fraction of the cost</h4>
<ul>
<li>Exclusive to us - Puromycin expressing LentiORF library barcoded for pooled screening applications</li>
<li>Each ORF Clone contains a unique 24 NT barcode sequence for easy deconvolution and identification of hits</li>
<li>Perform overexpression screening to study cancer phenotypes</li>
<li>Trusted TRC3 (The RNAi Consortium) content with ORF gene expression spanning 14,000+ human genes and 17,000+ clones</li>
<li>Simplified whole genome ORF screening in standard or difficult to expand cell lines</li>
</ul>


<h4>Survey the entire human kinome for novel hits with pooled CRISPR knockout clones</h4>
<ul>
<li>Over 6,000 unique lentiviral clones expressing both Cas9 and gRNA sequences designed to functionally inactivate (knockout) nearly 700 human kinase genes</li>
<li>High clone coverage (7-10 distinct gRNAs per kinase gene, average of 8.6 per gene) increases the statistical strength of your hit analysis</li>
<li>Numerous built-in enrichment and depletion controls</li>
<li>The pool contains 100 non-targeting control gRNAs as normalization standards</li>
<li>The human kinase pool is deep sequenced to verify clone representation and distribution</li>
<li>gRNA design and tiling was created with a stringent bioinformatics approach to minimize the potential for off-targeting</li>
</ul>


<h4>Knockdown the genome</h4>
<ul>
<li>Trusted TRC (The RNAi Consortium) content with shRNAs targeting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to us</li>
<li>Enjoy the power of lentiviral transduction for screening in standard or difficult to expand cell lines.</li>
<li>Deliverables: 50 μl of each pool at a minimum of 5x10<sup>8</sup> viral particles/ml (via p24), ships immediately</li>
<li>Versatile and compatible with FACS and most downstream fluorescent assays</li>
<li>Affordable and easy-to-use allowing for bench-top discovery without robotics or liquid handling</li>
</ul>


<h4>Knockdown and focus on the kinase gene pathway</h4>
<ul>
<li>Over 9,400 unique lentiviral shRNA clones to knockdown 740+ human kinase genes</li>
<li>High clone coverage (average of 12.6 clones per gene) increases the statistical strength of your hit analysis</li>
<li>Deliverables: 100 μl of each pool at a minimum of 5x10<sup>8</sup> viral particles/ml (via p24), ships immediately</li>
<li>Numerous built-in enrichment, depletion, and non-target control clones allows researchers to confidently gauge the success of their pooled screening experiments</li>
</ul>


aem-GridColumn aem-GridColumn--default--12

aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0 aem-GridColumn--default--none

title

text_1704268893_copy_1931534704

text_97621350_copy

button_copy

button_copy_copy

CRISPR Lentrivirus<br>Order - Our Pool

sectiondivider_copy

text_1704268893_copy_1048641882

text_97621350_copy_c

button_copy_copy_365255693

DNA<br>Request Quote

button_copy_copy_cop

CRISPR Lentivirus<br>Request Quote

sectiondivider_copy_

text_1704268893_copy

text_97621350_copy_c_1833156622

button_copy_copy_365

CRISPR Lentivirus<br>Order

sectiondivider_copy__1005199895

text_1704268893_copy_318873389

text_97621350_copy_c_2142493279

button_copy_copy_365_1022829896

shRNA Lentivirus<br>Order

sectiondivider_copy__1965790559

text_1704268893_copy_1629986204

text_97621350_copy_c_1090947281

button_copy_copy_365_258193615

button_copy_copy_cop_1443472219

shRNA Lentivirus<br>Request Quote

sectiondivider_copy__2073191198

text_1704268893_copy_478486612

text_97621350_copy_c_299058476

button_copy_copy_365_1435521818

sectiondivider_copy__1226540533

text_1704268893_copy_69749030

text_97621350_copy_c_1728679208

button_copy_copy_365_793658029

ORF Lentivirus<br>Order

sectiondivider_copy__832754305

text_1704268893_copy_1000329829

text_97621350_copy_c_1442990492

button_copy_copy_365_531785657

button_copy_copy_cop_647492040

ORF Lentivirus<br>Request Quote

Button

Container component

Image

Static Table

Text

Title

Video

Lentiviral Pools Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF); Synonyms: CRISPR knockdown,Custom CRISPR Pooled Library,Custom ORF Lentiviral Overexpression Pool,Custom shRNA Pooled Library,Gecko2 Human Whole Genome CRISPR Pool,Gecko2 Mouse Whole Genome CRISPR Pool,Human Kinase Lentiviral CRISPR Pool,MISSION® Human Kinase shRNA Lentiviral Pool,MISSION® LentiPlex® Human Pooled shRNA Library,MISSION® LentiPlex®Mouse Pooled shRNA Library,MISSION® TRC3 LentiORF Whole Genome Lentiviral Pool,Whole Human Genome Lentiviral CRISPR Pool at Sigma-Aldrich

Lentiviral Pools Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF) | Sigma-Aldrich

Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

Lentiviral Pools Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

Lentiviral Pools

Synonym: CRISPR knockdown, Custom CRISPR Pooled Library, Custom ORF Lentiviral Overexpression Pool, Custom shRNA Pooled Library, Gecko2 Human Whole Genome CRISPR Pool, Gecko2 Mouse Whole Genome CRISPR Pool, Human Kinase Lentiviral CRISPR Pool, MISSION® Human Kinase shRNA Lentiviral Pool, MISSION® LentiPlex® Human Pooled shRNA Library, MISSION® LentiPlex®Mouse Pooled shRNA Library, MISSION® TRC3 LentiORF Whole Genome Lentiviral Pool, Whole Human Genome Lentiviral CRISPR Pool

Key Documents

Lentiviral Pools

Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

About This Item

Recommended Products

related product

SHPH01

SHPM01

SHPHLIBR

SHPMLIBR

HKCRISPR

SHK001

Customers Also Viewed

Technical Service

Key Documents

Lentiviral Pools

Knockdown (RNAi), Knockout (CRISPR), and Express (LentiORF)

About This Item

Recommended Products

Related Products

related product

SHPH01

SHPM01

SHPHLIBR

SHPMLIBR

HKCRISPR

SHK001

Customers Also Viewed

Technical Service