L9381

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

FITC conjugate, lyophilized powder

• Synonym(s): Bandeirea simplicifolia agglutinin, BS-I
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: Bandieraea simplicifolia
• Quality Level: 200
• conjugate: FITC conjugate
• form: lyophilized powder
• potency: <100 μg per mL agglutination activity (using human blood group A erythrocytes)
• composition: Protein, ~10% Lowry
• extent of labeling: 1-3 mol FITC per mol protein
• storage temp.: −20°C

Description

Biochem/physiol Actions

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B₄, BSI-AB₃, BSI-A₂B₂, BSI-A₃B and BSI-A₄. BSI-B₄ is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A₄ has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Physical form

Contains phosphate buffer salts and sodium chloride

Analysis Note

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves