L7533

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

• Synonym(s): LB broth
• UNSPSC Code: 41106200
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• form: powder
• composition: Agar, 15 g/L ; NaCl, 5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• technique(s): microbiological culture: suitable
• application(s): agriculture; food and beverages; microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox EZMix powder with agar provides:

• A budget-friendly alternative to pre-poured plates
• Granulated, dust-free format for safer handling and faster mixing
• Convenient small package to eliminate weighing
• Easy scale-up using larger package sizes
• Standard formulation

Preparation Note

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Reconstitution

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable