I7901
Monoclonal Anti-Interleukin-6 antibody produced in mouse
clone 6708.11, purified immunoglobulin, lyophilized powder
Synonym(s):
Anti-IL-6
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6708.11, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
indirect ELISA: suitable
neutralization: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IL6(3569)
Related Categories
General description
Interleukin-6 is a pleotropic cytokine involved in hematopoiesis, inflammation and immune response. IL-6 is secreted by macrophages, dendritic cells and B cells, in response to TNF-α, PGDF and IL-1. The signaling by IL-6 is mediated by heterodimeric receptor, IL-6R, comprising of IL-6Rα (soluble factor) and gp130 (sometimes called IL-6Rβ). gp130 is crucial component for downstream signal transduction. In response to IL-6, gp130 activates kinases of JAK family, STATs, MAPK and Ras proteins. IL-6-mediated signaling is essential for normal development of T and B cells and has been implicated in many diseases such as rheumatoid arthritis, Crohn disease, sepsis, fever, systemic lupus erythematosus, osteoporosis and insulin resistance. Monoclonal Anti-Interleukin-6 antibody recognizes human IL-6. The antibody shows no cross-reactivity with recombinant mouse IL-6 or recombinant human IL-6 sR, OSM, LIF CNTF, or IL-11 or recombinant horse gp130.
Specificity
The antibody will neutralize the biological activity of recombinant human IL-6. The antibody shows no cross-reactivity with recombinant mouse IL-6 or recombinant human IL-6 sR, OSM, LIF CNTF, or IL-11 or recombinant horse gp130.
Immunogen
recombinant human IL-6.
Application
Monoclonal Anti-Interleukin-6 antibody may be used for ELISA at a minimum antibody concentration of 4 μg/ml. For immunoblotting a working antibody concentration of 1-2 μg/ml may be used. The antibody may be used in neutralization assays; ND50 is 0.01-0.15 μg/ml. For neutralization of IL-6 from human peripheral blood mononuclear cells, a concentration of 2.5 μg/ml was used.
Physical form
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.
Preparation Note
Purified using protein A.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
The novel differentiation of human blood mononuclear cells into CD1a-negative dendritic cells is stimulated in the absence of exogenous cytokines by an extract prepared from pinecones
Bradley WG et al
Immunopharmacology, 3, 209-223 (2003)
Siti-Fauziah Hendrayani et al.
Oncotarget, 7(27), 41974-41985 (2016-06-02)
The IL-6/STAT3/NF-κB positive feedback loop links inflammation to cancer and maintains cells at a transformed state. Similarly, cancer-associated myofibroblats remains active even in absence of cancer cells. However, the molecular basis of this sustained active state remains elusive. We have
Mysoon M Al-Ansari et al.
Oncotarget, 9(78), 34681-34690 (2018-11-10)
The ATR protein kinase is a master regulator of the cellular responses to DNA damage and replication stresses. Despite these crucial physiological roles, the implication of ATR in human carcinogenesis remains elusive. We have shown here that the ATR level
David S Hong et al.
Cancer, 110(9), 1911-1928 (2007-09-13)
Interleukin-6 (IL-6) plays a major role in the response to injury or infection and is involved in the immune response, inflammation, and hematopoiesis. Its deregulation impacts numerous disease states, including many types of cancer. Consequently, modulating IL-6 may be an
Interleukin-6-type cytokines.
P B Sehgal et al.
Annals of the New York Academy of Sciences, 762, 1-14 (1995-07-21)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service