Skip to Content
Merck
All Photos(1)

Documents

H9914

Millipore

HIS-Select® Nickel Magnetic Agarose Beads

Synonym(s):

nickel charged magnetic beaded agarose

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

conjugate

magnetic beads

Quality Level

200

form

suspension

shelf life

2 yr (Unopened product)

matrix

6% beaded magnetic agarose

capacity

≥15 mg/mL binding capacity

storage temp.

2-8°C

Related Categories

General description

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Application

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Physical form

Supplied as a 50% slurry suspension in 30% Ethanol.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

86.0 °F

Flash Point(C)

30 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service