GE27-9261-01

First-strand cDNA Synthesis Kit

Cytiva 27-9261-01

• UNSPSC Code: 41116006
• NACRES: NA.55

Properties

• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• manufacturer/tradename: Cytiva 27-9261-01
• color: colorless
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.

First-Strand cDNA Synthesis Kit is designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. Primers are not included in the preassembled First-Strand Reaction Mixes, thus allowing use of a primer of choice. Two primers are included with the kit. pd(N)6 Primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5′-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail.

The NotI-(dT)18 Bifunctional Primer can be used to prime selectively on mRNA that has a poly(A) tail. It can also be used as a PCR primer following first-strand synthesis. The NotI restriction site, located at the 5′-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products.

First-Strand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, pd(N)6 Primer, NotI-(dT)18 Bifunctional Primer, and instruction booklet.

Application

First-strand cDNA synthesis kit has been used to reverse transcribe total RNA into cDNA.

Features and Benefits

• For synthesis of single-stranded cDNA prior to amplification by PCR.
• Features preassembled bulk reaction mixes to save time and minimize potential for pipetting errors.
• Allows users to choose primers.
• Includes a choice of two primers: pd(N)6 random hexamers and NotI-(dT)18.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids